Abdul Fatah A. Samad, Nazaruddin Muhammad Ali, Ismanizan Ismail
In plants, a group of non-coding small RNA (sRNA) has been provento be an important player in regulating gene expression that can govern network of genetic systems. The two major classes of sRNA which are very extensively studied through deep sequencing, microRNA (miRNA) and small-interfering RNA (siRNA) classes, are well documented. However, the isolation method of sRNA differs depending on the type of sample. Here, we demonstrate the miRNA library preparation using two different Small RNA Library preparation kit, Illumina®TruSeq® Small RNA Preparation and NEBNext® Multiplex Small RNA Library Preparation kit on a plant rich in secondary metabolite Persicaria minor using recommended protocol. The result show NEBNext® Multiplex Small RNA Library Preparation kit can recover small RNA better than Illumina®TruSeq® Small RNA Preparation kit. Thus, this study recommended NEBNext® Multiplex Small RNA Library Preparation kit for miRNA library preparation on Persicaria minor. © 2015 Penerbit UTM Press. All rights reserved.
School of Bioscience and Biotechnology, Universiti Kebangsaan Malaysia, Bangi, 43600, Malaysia; Institute of Biology System, Universiti Kebangsaan Malaysia, Bangi, 43600, Malaysia; Department of Chemistry, University of Syiah Kuala, Darussalam-Banda Aceh, 23111, Indonesia
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