Rosnani Nasution, Tonel Barus, Pandapotan Nasution, Nurdin Saidi
Research on plant leaves Artocarpus camansi (Kulu) was aimed to find a chemical compound with antidiabetic activity in male Swiss Webster mice. The study was began by preparing hexane, ethyl acetate and methanol extracts of the leaves of A. camansi plant. The antidiabetic activity assay revealed that hexane extract was most active extract. According to this, the hexane extract was further fractionated to give pure isolate. The pure isolate has a melting point of 77-80° C. Then the isolate was further characterized by ultra violet (UV), infra red (IR), one and two dimensional nuclear magnetic resonance (NMR) experiments such as 1HNMR, 13CNMR, distortionless enhancement by polarization transfer (DEPT), 1H-1H homo correlation spectroscopy (COSY), heteronuclear single quantum correlation (HSQC), and heteronuclear multiple bond coherence (HMBC), the result of the spectral analyses suggested that the isolate was β-sitosterol propionate. In the antidiabetic activity assays, β-sitosterol propionate showed, a greater ability to reduce blood glucose than crude extract (extract of hexane, ethyl acetate extract and methanol extract), that is,30 minutes after the administration of β-sitosterol propionate can reduce blood glucose of mice 87.67 mg/dL; after 60 minutes reduce the blood sugar as much as 89 mg /dL; and 90 minute, reduce blood sugar 22 mg /dL, in male Swiss Webster mice. Analysis of this variance were performed using ANOVA one way Post hoc analysis procedures, significant differences (p<0.05) and (p<0.01) conducted using Tukey.
Department of Chemistry, Syiah Kuala University, Banda Aceh 23111, Indonesia; Department of Chemistry, North of Sumatera University, Medan, Indonesia
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