Isolation and Characterization of Indigenous Rizobacteria from Shallot (Allium ascalonicum, L) Rizosphere as PGPR Agents

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R. Husna, Hasanuddin, R. Sriwati, Sulastri, Nurachidan, A. Arini, U. Wulandari

2025 IOP Conference Series: Earth and Environmental Science Vol. 1476 Issue 1 Conference paper Cited by 2 SDG 15SDG 17 Quartile

Abstract

The one microorganisms that function as biological agents and many colonize the rhizosphere of plants is rhizobacteria. The study aimed to isolate and characterize rhizobacteria from the shallot rhizosphere that function as Plant Growth Promoting Rhizobacteria (PGPR) agents. Soil samples were taken from the root zone of shallot plants in Glumpang Baroe and Blang Bambie villages, Pidie, Aceh Province, Indonesia. The study employed quantitative and qualitative descriptive analysis methods, utilizing the serial dilution technique.. Macroscopic, microscopic (gram test), and phosphate solubilization test parameters were observed. Macroscopically, there was a diversity of rhizobacterial isolates based on shape, color, edge and elevation. Based on gram test, Twenty rhizobacterial isolates were found in samples from Glumpang Baro (GB) (7 gram positive and 13 gram negative isolates), from Blang Bambie (BB) 11 isolates (7 gram positive and 4 gram negative isolates). Nineteen isolates from GB and eleven isolates from BB were able to dissolve phosphate, only one GB isolate was not able to. The rhizobacterial isolate (GB 6/1) produced the highest PSI (2.80) and the lowest isolate (GB 7/2) (2.33), while the rhizobacterial isolate (BB 4/2) produced the highest PSI value (2.73) and the lowest (2.40) in isolates (BB 4/1 and BB 7/3). © 2025 Institute of Physics Publishing. All rights reserved.

Affiliations

Faculty of Agriculture, Syiah Kuala University, Jalan Tgk. Hasan Krueng Kalee 3, Darussalam-Banda, Aceh, 23111, Indonesia; Department of Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Jalan Tgk. Hasan Krueng Kalee 3, Darussalam-Banda, Aceh, 23111, Indonesia; Department of Plant Protection, Faculty of Agriculture, Syiah Kuala University, Jalan Tgk. Hasan Krueng Kalee 3, Darussalam-Banda, Aceh, 23111, Indonesia; National Research and Innovation Agency, Cibinong Science Center, Jalan Raya Jakarta - Cibinong-Bogor, Bogor, 16915, Indonesia; Student of Department Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Jalan Tgk. Hasan Krueng Kalee 3, Darussalam-Banda, Aceh, 23111, Indonesia

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