Fildzah Fildzah, Suhartono Suhartono, Ria Ceriana, Rosnizar Rosnizar
Green cincau leaves, also known as Premna oblongifolia, have various therapeutic uses owing to their medicinal properties. This plant contains abundant bioactive substances, particularly flavonoids and polyphenols. Several studies have reported its anti-inflammatory, anticancer, and antioxidant properties. However, the effect of green cincau extract (GCE) on the proliferation of MSC-like cells and its effect on cell morphology are not yet known. This study aimed to investigate the effects of P. oblongifolia Merr. extract on the proliferation and morphology of mesenchymal stem cell-like (MSC-like) cells derived from rat bone marrow. Rat bone marrow was harvested, and cells were isolated and cultured in modified Dulbecco's Modified Eagle's Media (mDMEM). The initial cell number in the first subculture was recorded, followed by GCE treatment at concentrations of 0, 150, 600 and 1200 μg/mL for nine days. Cell counts were obtained at the beginning and end of the treatment period and the population doubling time (PDT) was calculated. The cell morphology was observed under an inverted microscope on days 3, 7 and 9. The results indicated a significant increase in cell counts following GCE treatment in contrast to the control group. The findings at a concentration of 600 μg/mL showed the shortest PDT of 5.67±1.90 days along with the highest average final cell count of 1.87×105 cells. The cell morphology remained largely unchanged, with cells continuing to exhibit a spindle shape, resembling fibroblasts. This study offers initial insights into the use of the extract as a promoter of cellular differentiation in stem cell research and enhances our knowledge of plant-derived compounds in regenerative medicine. © Published under licence by IOP Publishing Ltd.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Aceh, Banda Aceh, 23111, Indonesia; Academy of Pharmacy, YPPM Mandiri, Aceh, Banda Aceh, Indonesia
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