Z.A. Muchlisin, R.R. Hasibuan, S. Maulida, K. Eriani, N. Fadli, A.A. Muhammadar, L.S. Handayani, I. Suharman, M. Kocabas, F.K. Kocabas
Nile tilapia Oreochromis niloticus is a popular freshwater fish that has been extensively and intensively cultured worldwide. However, cryopreservation of its sperm, especially pre-freezing procedure, has not been properly developed. Therefore, the study aimed to determine the best pre-freezing procedure for cryopreservation of Nile tilapia Oreochromis niloticus sperm. The completely randomized design with five treatments and four replications was employed in this study. The tested treatments were T1=4°C → 0°C →-4°C →-10°C →-79°C →-196°C, T2=4°C→ 0°C →-4°C →-10°C →-196°C, T3=4°C → 0°C →-4°C →-196°C, T4=4°C → 0°C →-196°C), and T5=4°C →-196°C, with a 10 min equilibration at each respective temperature. Furthermore, sperm was cryopreserved for two weeks in liquid nitrogen (-179oC). The results of the ANOVA test showed that pre-freezing had a significant effect on sperm motility, and viability (P<0.05), but had no considerable impact on sperm abnormality (P>0.05). Treatment T4 exhibited higher motility and viability, but these values were not significantly different from T3 and T5. Based on practical consideration, it was recommended to utilize the T5 pre-freezing procedures (4°C →-196°C) for cryopreservation of Nile tilapia sperm. Considering these results, Nile tilapia sperm can be directly cryopreserved into liquid nitrogen after equilibration at 4°C for 10 min. © 2024 The Authors.
Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia; Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia; Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Universitas Riau, Pekanbaru, Indonesia; Faculty of Life Sciences, Karadeniz Technical University, Trabzon, Turkey; Faculty of Fisheries, Munzur University, Turkey
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