Meutia Maulina, Dessy Rakhmawati Emril, Endang Mutiawati, Etriwati Etriwati, Sri Hastuti, Safrizal Rahman, Zulkarnain Zulkarnain
Peripheral nerve damage is a common medical problem that frequently leads to significant functional limitations and long-term disabilities. Platelet-rich plasma (PRP), a growth factor-rich substance, promotes axonal regeneration in peripheral nerve injuries. This study aimed to investigate the effect of PRP on axonal regeneration in Wistar rats (Rattus norvegicus) after peripheral nerve injury via histomorphometric analysis. This study used a post-test-only control-group design. Twenty-five white rats were randomly assigned to the control or treatment groups (n=5). The control group consisted of the sham-operated and saline groups. The treatment groups consisted of local, intraperitoneal, or combined PRP. A single dose of PRP was administered after axonotmesis of the sciatic nerve. After 21 days, a surgical procedure was performed to extract sciatic nerve tissue, which was then stained with Luxol fast blue for histomorphometric analysis. The mean diameter of the fibers, diameter of the axons, number of axons, and myelin sheath thickness were analyzed using MANOVA (p<0.05). Compared with the saline group, the PRP treatment group presented a greater number of regenerating myelinated axons with larger fiber and axon diameters, and thicker myelin (p<0.05). The myelinated axons in the sham-operated group showed a typical morphology, whereas those in the saline group showed a decrease in myelinated axons, smaller fiber and axon diameters, and thinner, irregular myelin sheaths. Injection of a combination of PRP, both intraperitoneally and locally, promoted the regeneration of damaged nerves in rats. © 2024 Ondokuz Mayis Universitesi. All rights reserved.
Department of Histology, Faculty of Medicine, Universitas Malikussaleh, Aceh, Indonesia; Department of Neurology, Faculty of Medicine, Universitas Syiah Kuala-Dr. Zainoel Abidin Hospital, Aceh, Indonesia; Department of Pathology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Aceh, Indonesia; Department of Orthopedic and Traumatology, Faculty of Medicine, Universitas Syiah Kuala-Dr. Zainoel Abidin Hospital, Aceh, Indonesia; Department of Physiology, Faculty of Medicine, Universitas Syiah Kuala, Aceh, Indonesia
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