Cut Intan Novita, Kartini Eriani, Amalia Sutriana, Tongku Nizwan Siregar, Ni Wayan Karja
Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) hormones are often added to ovarian vitrification media to prevent apoptosis of granulosa cell follicles. Bovine pituitary has been proven as an effective substitute for the functions of FSH and LH in some studies related to superovulation due to its high content of both hormones. Therefore, this study aimed to determine the effect of bovine pituitary extract (BPE) supplementaion to vitrification media. A total of 18 ovaries were usedwhich was divided into two groups: the vitrification medium group without Bovine Pituitary Extract (BPE, GibscoTM) (1 ml PBS + 0.5 M sucrose + 30% ethylene glycol) and with BPE addition (1 ml PBS + 0.5 M sucrose + 30% ethylene glycol + BPE 30 μg/mL). Each group was separated into three vitrification times of 0, 7, and 14 days with three replications. The results showed that adding BPE to the vitrification medium increased the number of intact follicles (P<0.05) at each development stage, with the highest number occurring in the primordial and primary follicle stages. The number of primordial and primary follicles on day 14 of vitrification the control group vs the BPE group was 62.08±18.25 vs 72.72±5.67 and 45.62±15, 9 vs 65.78±7.17, respectively. In conclusion, the addition of BPE to the vitrification medium could increase the number of intact follicles, preventing damage that may occur during the vitrification process. Copyright: 2024 by the authors. Licensee ResearchersLinks Ltd, England, UK. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh, Indonesia; Animal Sciences Study Program, Faculty of Agriculture, Universitas Syiah Kuala, Banda Aceh, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Indonesia; Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Indonesia; Laboratory of Reproduction, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Indonesia; Research Center of Aceh Cattle and Local Livestock, Faculty of Agriculture, Universitas Syiah Kuala, Indonesia; Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia
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