A. Sofia, E.M. Sari, M.A.N. Abdullah, Gholib, S. Wahyuni
The objectives of this research were to the identification of GHRH gen in Gayo buffalo using the PCRRFLP method. GHRH is a hypothalamic hormone which stimulates growth hormone secretion in the pituitary gland. Growth Hormone Releasing Hormone is a hormone that stimulates the synthesis and secretion of Growth Hormone which influences growth. Therefore, the GHRH gene is a genetic characteristic that can be used as a basis for selecting livestock. The objective of this study was to identify polymorphisms Growth Hormone-releasing hormone (GHRH) gene of the Gayo buffalo. A total of 15 blood samples from Aceh Tengah were used to determine polymorphism using the PCR-RFLP method and were detected using endonuclease HaeIII. The isolated DNA using a pair of primers 5’-GTA AGG ATG GCT CTG CCA GGT3 ‘and GHRH reverse 5’- TGC ATG ATG CTG TCC CTC TGG A-3’ produced fragments as long as 451 bp. In this study, there were GHRH/HaeIII genotypes AA genotypes 100%, AB genotype 0% and BB genotype 0%. It could verify that the Gayo buffalo is monomorphic. © 2024 selection and editorial matter, Purnaning Dhian Isnaeni, Mashitah Shikh Maidin, Muh. Amrullah Pagala, Widhi Kurniawan and Asma Bio Kimestri; individual chapters, the contributors.
Department of Animal Science, Faculty of Agriculture, Syiah Kuala University, Indonesia; Department of Animal Science, Faculty of Agriculture, Syiah Kuala University, Darussalam, Aceh Cattle and Local Livestock Research Center, Indonesia; Faculty of Veterinary, Syiah Kuala University, Indonesia
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