Luvi S. Handayani, Siti Maulida, Sri R. Rahayu, Nanda M. Razi, Mehmet Kocabas, Filiz K. Kocabas, Kartini Eriani, Nur Fadli, Moh N. Siti-Azizah, Kasi Marimuthu, Zainal A. Muchlisin
The study aims to determine the type of extender and the best dilution ratio for short preservation walking catfish Clarias batrachus sperm at a refrigeration temperature of 4°C. The study employed a completely randomized design with two factors, each consisting of 6 treatments and 3 replications. The experiments were divided into 2 stages: the first stage tested several types of extenders, namely tap water, Ringer’s solution, physiological solution, Alsever’s solution, urea solution, and glucose base solution, all at the same dilution level of 1:20 (sperm: extender, v/v). The second stage examined various levels of sperm dilution ratios, namely 1:10, 1:20, 1:30, 1:40, 1:50, and 1:60 (sperm: extender, v/v). The samples were stored at a refrigerated temperature of 4°C. Furthermore, motility and viability were monitored at 24-hour intervals for next 6 days, and on the final day of the experiment. The ANOVA test showed that the type of extender and dilution ratio significantly affected the motility and viability of the spermatozoa and percentage of fertilization (P < 0.05). Generally, the quality of sperm decreased after preservation for 144 hours at 4°C, but diluted with Ringer solution at a ratio of 1:40 (sperm: extender, v/v), still maintains good parameters. Therefore, it is recommended that Ringer’s solution at a dilution ratio of 1:40 is suitable for preserving walking catfish C. batrachus sperm at a refrigerated temperature of 4°C. © 2024 University of Warmia and Mazury. All rights reserved.
Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh, Indonesia; Department of Wildlife Ecology and Management, Faculty of Forestry, Karadeniz Technical University, Trabzon, Turkey; Fisheries Faculty Munzur University, Tunceli, Turkey; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh, Indonesia; Department of Aquaculture, Faculty of Marine, Fisheries Universita Syiah Kuala, Banda Aceh, Indonesia; Institute of Marine Biotechnology and Climate Adaptation, University Malaysia, Terengganu, Malaysia; Department of Environmental Sciences, Tezpur Central University, Tezpur, 784028, India
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