Molecular identification and phylogenetic reconstruction of marine fish from Kutaradja fish landing Station, Banda Aceh; [Identificación molecular y reconstrucción filogenética de peces marinos de la estación de desembarque de pescado de Kutaradja, Banda Aceh]

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S. Andriyono, A. Damora, A.A. Hidayani

2024 Archivos de Zootecnia Vol. 73 Issue 283 Article Cited by 0 SDG 14SDG 17 Quartile

Abstract

The large potential of marine resources possessed by Banda Aceh Province is expected to be utilized optimally. Complete identification of marine fish resources is an important requirement in order to support their utilization and preservation in Banda Aceh Province. In this study, a molecular identification approach is carried out in addition to conducting a morphological identification that has been commonly used. In this molecular identification study, the target DNA is the Cytochrome c oxidase subunit I (COI) region located in the mitochondria. The extraction method used a DNA Kit that was adjusted to the manufacturer’s guidelines, then continued with PCR to duplicate the extracted DNA. The PCR results were carried out by direct sequencing and sequence analysis was performed using Chromas and Mega7 software. The results obtained were 47 COI sequences were generated representing 33 genera, 19 families, and 5 orders. Perciformes was the most predominant group of fish, and several fish species with important economic value were identified such as the Scombridae group including Thunnus albacares, Auxis thazard, and Katsuwonus pelamis. From the resulting sequence, there are one haplotype from the Scombridae family, two haplotypes from the Carangidae family, and two haplotypes from the Serranidae family. This study is essential in fisheries biology studies and other fisheries studies to support the sustainable utilization of marine fisheries potential in Banda Aceh. © 2024, UCOPress. Editorial Universidad de Cordoba. All rights reserved.

Affiliations

Department of Marine, Faculty of Fisheries and Marine, Universitas Airlangga, Jl. Mulyorejo Kampus C, East Java, Surabaya, 60115, Indonesia; Faculty of Marine and Fisheries, Universitas Syiah Kuala, Jl. Putroe Phang, Aceh, Darussalam, Banda Aceh, 23111, Indonesia; Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University, Jl. Perintis Kemerdekaan KM. 10, South Sulawesi, Makassar, 90245, Indonesia

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