Vivi Mardina, Wahyuni Rizki, Sukmawan F. Santosa, Nurdin, Febriani, Teuku M. Iqbalsyah
Cellulase is one of the enzymes that play a role in degrading lignocellulosic biomass. The study objective was to optimize the cellulase fermentation conditions by a bacterium isolated from undersea fumaroles, dubbed PLS 75. The conditions include incubation time, temperature, pH, and salt content. Cellulase was produced in ½ Thermus liquid medium with 1% Carboxy Methyl Cellulose (CMC) as the inducer. The cellulosic activity was measured using the 3,5-dinitrosalicylic acid (DNS) method. A qualitative screening showed that PLS 75 could produce cellulase, indicated by a clear zone after staining with Congo red. The growth of PLS 75 during fermentation was monitored for 30 hours. Further optimization of the cellulase production was carried out by varying fermentation temperature (60, 65, 70 and 75 °C), pH (5; 6; 7; 8 and 9), and salt concentration (0.6; 1.1; 1.5; and 3 M). The optimum cellulase activity was produced at 20 hours, pH 7, temperature 70 °C, and salt content of 0.6 M, with an average activity of about 6.6 U/mL. The results showed that PLS 75 was a moderate halophilic microorganism. Its capability to produce cellulase may be developed further to be applied in cellulose-degrading industries. © 2023 Author(s).
Biomolecules Application Research Group, Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia; Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh, 723111, Indonesia
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