Zulkarnain Zulkarnain, Zulvikar Syambani Ulhaq, Hidayat Sujuti, Djoko Wahono Soeatmadji, Hendra Zufry, Dyah Kinasih Wuragil, Agung Pramana W. Marhendra, Wibi Riawan, Siti Kurniawati, Yudit Oktanella, Aulanni’am Aulanni’am
Background: Graves’ disease (GD) is an autoimmune disease, and it accounts for major cases of hyperthyroidism. Antibody against thyroid-stimulating hormone receptor/TSHR (TRAb) is responsible for hyperthyroidism and is considered as a diagnostic marker for GD. Therefore, we developed a recombinant protein of human TSHR-169 (hTSHR-169), which was specifically recognized TRAb in the serum of GD patients and then compare the diagnostic performance between ELISA and dot blot of TRAb tests for their ability to diagnose GD. Methods: 20 GD patients and 20 healthy individuals from the Indonesian population were enrolled. TRAb concentration and density were quantified. Comparative analysis was performed using receiver-operating curve (ROC) analysis. Results: For dot blot assay, the minimum concentration to detect TRAb requiring 100 ng of antigen with antiserum diluted at 1:60. For diagnosing GD, the ELISA yielded a higher AUC compared with the dot blot assay (0.95 and 0.85, respectively). Using the recommended cutoff values, the efficiency of both assays was examined by comparing the specificity and sensitivity of the assays to the clinical diagnosis. The ELISA showed 80% and 95%, while the dot blot assay showed 70% and 95% sensitivity and specificity, respectively. Conclusion: Although the dot blot assay exhibited lower performance than the ELISA method, the dot blot assay is a simple and rapid diagnostic assay that is suitable for diagnosing GD in rural areas, in which healthcare facilities sometimes are not accessible. © 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.
Faculty of Medicine, Syiah Kuala University, Banda Aceh, Indonesia; Faculty of Medicine, Brawijaya University, Malang, Indonesia; National Research and Innovation Agency (BRIN), Jakarta, Indonesia; Faculty of Medicine and Health Sciences, Maulana Malik Ibrahim State Islamic University of Malang, Batu, Indonesia; Internal Medicine Department, Saiful Anwar General Hospital, Malang, Indonesia; Faculty of Veterinary Medicine, Brawijaya University, Malang, Indonesia; Faculty of Science, Brawijaya University, Malang, Indonesia
Research at a Glance
Register to unlock
Topics & SDG Alignment
Register to unlock
Collaboration
Register to unlock
Author Profile (Selected)
Register to unlock
References Overview
Register to unlock
Journal & Source
Register to unlock
Metadata & Integrity
Register to unlock