Dharli Syafriza, Ayudia Rifki, Vera Yulina, Basri A. Gani
To examine the stress response and metabolic changes in S. mutans cells following contact with saliva that has been linked to growth and ionic characteristics. The saliva and S. mutans isolate ATCC 25175 were employed in this work. A spectrophotometer was used to measure growth. The ionic changes in saliva were determined using an ionic meter. Meanwhile, FTIR was used to examine S. mutans’ stress response and metabolic alterations (Fourier Transform Infra-Red). At the 24 hours, salivary growth response was 12.5% against S. mutans (100%), 25% saliva (82%), saliva 6.25%, and CHX (97%). At the incubation time of 48 and 72 hours, 12.5% and 6.25% of saliva had a better growth response to S. mutans (81-87%) than other saliva concentrations. Salivary concentrations of 12.5% and 6.25% can reduce the stress response of S. mutans by 54% and 64%, respectively) from the stress response to the tolerance of S. mutans (71%). Saliva 6.25% affected the cellular metabolism of S. mutans (15%) compared to saliva concentrations of 50 (16%), 25 (17%), and 12.5 (18%). S. mutans cell affinity metabolism, which is influenced by saliva and environmental factors, causes acid challenge activity to reach 245%, metabolic changes (102%), amino acid and nucleotide (88%), protein (73%), nucleic acid (69%), carbohydrates (17%), and lipids (6%). Saliva and its ionic properties can reduce and control the growth of S. mutans which is characterized by a decrease in the stress response of S. mutans, an increase in the growth response of saliva and cellular metabolism, and the metabolic affinity of S. mutans cells. © 2022, Journal of International Dental and Medical Research. All Rights Reserved.
Department of Pediatric Dentistry, Dentistry Faculty, Universitas Syiah Kuala, Darussalam, Aceh, Banda Aceh, Indonesia; Department of Oral Biology, Dentistry Faculty, Universitas Syiah Kuala, Aceh, Darussalam, Banda Aceh, Indonesia
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