Cut Intan Novita, Rosna Sari, Eka Meutia Sari
This research aimed to know the effect of the addition of jamblang leaves extract (Syzygium cumini) in skim milk-egg yolk extender on motility, abnormality, and viability of Aceh cattle spermatozoa stored at 4ºC. The research design used was Complete Randomized Design consisting of 5 treatments and 5 replications. The treatments consisted of J0 (egg yolk-skim milk), J1 (egg yolk-skim milk + jamblang leaf extract 0.2 g/100 ml), J2 (egg yolk-skim milk + jamblang leaf extract 0.4 g/100 ml), J3 (egg yolk-skim milk + jamblang leaf extract 0.6 g/100 ml) and J4 (egg yolk-skim milk + jamblang leaf extract 0.8 g/100 ml). The data related to fresh semen characteristics were analyzed descriptively, while spermatozoa quality following dilution and equilibration were analyzed using analysis of variance (ANOVA). The difference between treatment were compare using the Duncan’s multiple range test. The results indicated that fresh semen quality was still met the requirement of semen processing for freezing. Moreover, the addition of jamblang leaves extract in skim milk-egg yolk extender significant effect (P<0.05) on spermatozoa motility after equilibration and spermatozoa viability after dilution, while for spermatozoa motility after dilution, spermatozoa viability after equilibration, abnormal spermatozoa after dilution and equlibration did not have a significant effect. Based on result, it can be concluded that the addition of jamblang leaves extract in skim milk-egg yolk extender could maintain the quality of Aceh cattle spermatozoa, while the use of incorrect dose becomes toxic and disrupt the spermatozoa activities and leading to death. © 2021, Gadjah Mada University. All rights reserved.
Department of Animal Science, Faculty of Agriculture, Universitas Syiah Kuala, Indonesia Research Centre of Aceh Cattle, and Local Animal, Banda Aceh, Indonesia
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