Determination of Mercury Ions Level in River Water Using an Arduino Uno-Based TCS3200 Color Sensor with Silver Nanoparticle Indicators Synthesized from Moringa Oleifera Leaf Extract

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Khairi Suhud, Sri Rahayu Ningsih, Muhammad Syukri Surbakti, Muhammad Daffa Hadistya, Sitti Saleha, Yusnaidar, Siti Aishah Hasbullah, Ahmad Fairuz Bin Omar, Fathurrahmi, Muhammad Rifki Fauzan Damanik, Andriy Anta Kacaribu

2025 Trends in Sciences Vol. 22 Issue 8 Article Cited by 2 SDG 17SDG 6 Quartile

Abstract

Mercury ions (Hg2+) are highly toxic due to their neurotoxic properties. They pose significant risks to organisms and humans, especially when they accumulate in the body and frequently contaminate water. An Arduino Uno-based TCS3200 color sensor utilizing silver nanoparticles (AgNPs) as indicators offer a promising alternative for detecting Hg2+. This approach is characterized by its simplicity, cost-effectiveness, environmental friendliness, and effectiveness for real-time detection in resource-limited areas. This study synthesized AgNPs from the ethyl acetate extract of Moringa oleifera leaves. These AgNPs were tested for selectivity against various metals commonly found in aquatic environments. Once the selective metals were identified, sensitivity and selectivity tests were conducted to confirm that the sensor accurately measures specific analytes, even in the presence of other components within a sample. The results showed that the synthesized AgNPs are highly selective and specific for Hg2+ ions, achieving an accuracy range of 89.3-110 %. The sensor can detect Hg2+ ions with a detection limit (DL) of 0.069 ppm. Analysis of river water samples revealed Hg2+ concentrations ranging from 0.128 to 0.287 ppm. Hg2+ concentration was measured using a TCS3200 color sensor and validated against UV-Vis spectrophotometry. This confirmed its comparable results and effectiveness for accurate Hg2+ detection in real-world water samples and resource-limited areas. © 2025, Walailak University. All rights reserved.

Affiliations

Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia; Department of Physics, Faculty of Mathematics and Natural Science, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia; Department of Chemistry, Faculty of Science and Technology, Universitas Jambi, Jambi, 36361, Indonesia; Department of Chemistry, Faculty of Science Technology, Universiti Kebangsaan Malaysia, Selangor, 43600, Malaysia; School of Physics, Universiti Sains Malaysia, USM, Penang, 11800, Malaysia; Department of Biology Education, Faculty of Teacher Training and Education, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia; Doctoral Program of Agricultural Science, Postgraduate School, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia

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