Zuyasna, Ainun Marliah, Adinda Rahayu, Erita Hayati, Rika Husna
This study aims to determine the best colchicine concentration and immersion time in the colchicine induction process to obtain the highest level of diversity. The research was conducted at Sektor Timur Experimental Garden 2, Faculty of Agriculture, Syiah Kuala University, Darussalam, Banda Aceh, which was conducted from June to August 2020. This research used a factorial Randomized Complete Block Design (RCBD) with two factors. The first factor is the concentration of colchicine solution with 4 levels (0%, 1%, 2%, 3%) and the second factor is the immersion time with 4 levels (2, 4, 6, and 8 hours). There were 16 treatment combinations with each treatment repeated 4 times, and therefore 64 experimental units obtained. Parameters observed included increase in plant height, increase in diameter, number of branches, number of shoots, number of leaves, length of branches and leaf surface area. The results showed that the colchicine concentration had a significant effect on the surface area of the leaves after treatment (90 HSP), with the highest average occurring at the 3% colchicine concentration treatment. The duration of immersion with colchicine did not significantly affect all the variables observed. The combination of colchicine concentration treatment with immersion time had a significant effect on the number of patchouli plant branches at the age of 30 HSP, where the highest number of branches was at 2% colchicine concentration with 6 hours of soaking time. © 2021, Panji Sakti University. All rights reserved.
Jurusan Agroteknologi Fakultas Pertanian, Universitas Syiah Kuala, Banda Aceh, Indonesia
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