Marianne Marianne, Mariadi Mariadi, Sony Eka Nugraha, Rosnani Nasution, Pran Nando Syuhada, Sylvia Pandiangan
Background: Curcuma heyneana (Valeton & Zijp.) or temu giring has various pharmacological activities. However, its hepatoprotective activity toward ethanol induction has never been carried out. Objectives: The objective of this research was to evaluate the hepatoprotective activity of the C. heyneana rhizome extract toward Wistar rats induced by ethanol. Methods: The research was initiated with the determination of curcuminoid content, total phenolic content, total flavonoid content, and characterization of extract using gas chromatography-mass spectrometry (GC-MS). Hepatoprotective activity was tested using the C. heyneana extract at doses of 50, 100, and 150 mg/kg with 5 g/kg ethanol as an inducer. Aspartate transaminase (AST), alanine transaminase (ALT), liver weight, and macroscopic and microscopic liver were used as parameters. Data were analyzed using analysis of variance (ANOVA). Results: The curcuminoid content of the extract was 1.18% (w/w). Total phenolic content of the C. heyneana extract was 400.37 mg gallic acid equivalent (GAE)/g sample, while total flavonoid content was 27.25 mg quercetin equivalent (QE)/g sample. Nine compounds were identified in the extract. Administration of the extract at doses of 50, 100, and 150 mg/kg kept the liver normal. It was identified macroscopically from the dark red color without any white spot and normal liver weight. Furthermore, at doses of 50, 100, and 150 mg/kg, the extract inhibited AST and ALT elevation, which was significantly different from the negative control group (P < 0.05). The extract also prevented hepatocyte injury that was seen microscopically. Conclusions: It can be concluded that the C. heyneana extract at doses of 50, 100, and 150 mg/kg is effective as hepatoprotective in the liver injury induced by ethanol. Copyright © 2021, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
Department of Pharmacology Pharmacy, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia; Nanomedicine Centre, Universitas Sumatera Utara, Medan, 20155, Indonesia; Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia; Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Aceh, 23111, Indonesia; Undergraduate Students, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
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