Mingzhu Liu, Qing Yu, Yi Yi, Hehe Xiao, Dedi Fazriansyah Putra, Ke Ke, Qin Zhang, Pengfei Li
Grouper iridovirus was isolated from the diseased hybrid grouper in Guangxi province, China (SGIV-Gx), which has a high mortality rate in cultured groupers. Effective medicines for controlling SGIV-Gx infection are urgently needed. Lonicera japonica Thunb. (L. japonica) is a well-known medicinal plant. The application of L. japonica components against SGIV-Gx infection was assessed in this study. The safe, appropriate working concentration of each L. japonica component was identified (L. japonica aqueous extracts LAE ≤ 10 mg/mL; chlorogenic acid, CGA ≤ 250 μg/mL; cryptochlorogenic acid, CCGA≤500 μg/mL; isochlorogenic acid A, IAA ≤ 250 μg/mL; isochlorogenic acid B, IAB ≤ 250 μg/mL; isochlorogenic acid C, IAC ≤ 500 μg/mL; caffeic acid, CA ≤ 125 μg/mL; luteolin (LT) and inositol (IS), no cytotoxic effects even at 2000 μg/mL) in vitro and in vivo. The inhibitory activities of each L. japonica component against SGIV-Gx infection were analyzed by three methods: light microscopy, Q3-AFMP and RT-qPCR, which proved that, some L. japonica components (LAE, IAA, IAB, IAC, CA, LT, IS) exhibited the antiviral activity against SGIV-Gx infection in a dose-dependent manner, and LAE, IAA, IAB, IAC, CA, LT had the best antiviral activity against SGIV-Gx infection, with above 90% of inhibitory percentage. Overall, the results in this study suggest that L. japonica components have antiviral effects, making this plant a promising candidate for developing effective medicines for preventing and controlling SGIV-Gx infection in farmed groupers. © 2019
Guangxi Key Lab for Marine Biotechnology, Guangxi Institute of Oceanography, Guangxi Academy of Sciences, Nanning, China; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, School of Marine Science and Biotechnology, Guangxi University for Nationalities, Nanning, China; Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning, China; Guangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou, China; Guangxi Key Laboratory of Marine Environmental Science, Guangxi Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning, China; Department of Aquaculture, Syiah Kuala University, Banda Aceh, Indonesia
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