Teuku Reza Ferasyi, Mahdi Abrar, Ismail Ismail, Wahyu Eka Sari, Azhari Azhari, Mustafa Sabri, Faisal Jamin, Erwin Erwin, Siti Rani Ayuti, Mirni Lamid
Background and Aim: Multidrug-resistant (MDR) Escherichia coli in poultry poses a critical threat to food safety and public health. While studies have assessed resistance at the farm level, limited attention has been given to informal market environments that connect animals, humans, and surfaces. This study applied a One Health triangulation sampling approach to investigate the occurrence and antimicrobial resistance (AMR) profiles of E. coli isolated from broiler chickens and associated environments in traditional markets in Aceh Besar District and Banda Aceh City, Indonesia. Materials and Methods: A cross-sectional study was conducted in three traditional markets (Lambaro, Al-Mahirah, and Seutui). A total of 174 samples were collected, comprising fecal swabs (n = 54), chicken meat swabs (n = 54), poultry sellers’ hand swabs (n = 48), and chicken display table swabs (n = 18). Isolation of E. coli was performed using culture and biochemical confirmation. Antimicrobial susceptibility was tested using the Kirby–Bauer disk diffusion method against 11 antibiotics commonly used in veterinary and human medicine. Results: Overall, E. coli was isolated from 31.03% (54/174) of samples. Contamination was highest in fecal samples (13.79%), followed by chicken meat (8.62%), sellers’ hands (4.60%), and display tables (4.02%). Market-level prevalence was highest at Al-Mahirah (13.2%), followed by Lambaro (11.49%) and Seutui (6.32%). All isolates exhibited MDR phenotypes. Resistance was uniformly high to erythromycin (100%), tetracycline (94.6%), ampicillin (87.0%), and sulfamethoxazole/trimethoprim (83.3%), while lower resistance was observed to ciprofloxacin (23.8%) and gentamicin (20.2%). Distinct variation in resistance profiles was observed between markets and sample types. Conclusion: Traditional poultry markets represent critical hotspots for AMR dissemination at the human–animal–environment interface. Findings highlight the combined influence of unregulated antibiotic use in poultry production and inadequate hygiene practices on sustaining MDR E. coli. The triangulated One Health design demonstrates the added value of integrating animal, human, and environmental sampling for AMR surveillance. Strengthening antimicrobial stewardship, upgrading market hygiene infrastructure, and expanding integrated surveillance into national AMR monitoring frameworks are essential steps to mitigate public health risks. Copyright: Ferasyi, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/)
Laboratory of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia; Center for Tropical Veterinary Studies-One Health Collaboration Center, Universitas Syiah Kuala, Banda Aceh, Indonesia; Laboratory of Microbiology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia; Laboratory of Anatomy, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia; Laboratory of Clinics, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia; Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia; Division of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia
Research at a Glance
Register to unlock
Topics & SDG Alignment
Register to unlock
Collaboration
Register to unlock
Author Profile (Selected)
Register to unlock
References Overview
Register to unlock
Journal & Source
Register to unlock
Metadata & Integrity
Register to unlock