Syafruddin Syafruddin, Tongku Nizwan Siregar, Azrina Azrina, Teuku Armansyah, Budianto Panjaitan, Dwinna Aliza, Amalia Sutriana, Zuhrawati Zuhrawati, Rosmaidar Rosmaidar, Roslizawaty Roslizawaty
This research aimed to determine Aceh cattle ovarian follicle morphological integrity after vitrified by various cryoprotectants. Cryoprotectants used in this research were 30% ethylene glycol (EG), 30% dimethyl suphocide (DMSO), and combination of 15% EG + 15% DMSO. Prior to vitrification process, ovaries were cleansed by phosphate buffered saline (PBS) and then cut into ±1 mm³. Ovaries were consecutively submerged into the following liquid for 5 minutes each: PBS+ 0.25 M sucrose; PBS+ 0.5 M sucrose; PBS+ 0.5 M sucrose + 10% cryoprotectants; and PBS+ 0.5 M sucrose + 30% cryoprotectants. Then, ovaries were packed into straws with ±7 cm in length and ± 6 mm in diameter. Before kept in liquid nitrogen, ovaries were first exposed to nitrogen fume for 1 0 second. After being stored for 1 day, the ovaries were proceed for histological examination. The result showed that Aceh cattle ovarian follicle after vitrification using 30% EG yields the best morphological integrity. Cumulus oophorus, zona pellucida, granulose cell arrangement, theca interna, and theca externa cells were observed clearer in ovary which was vitrified with 30 % EG than those with 30% DMSO and combination of 15% EG + 15% DMSO. As conclusion, 30% EG was able to protect ovary morphological integrity better than 15 % EG + 15% DMSO and 30% DMSO. Furthermore, combination of 15% EG+ 15 % DMSO was relatively better in protecting ovary follicle morphological integrity compared to 30% DMSO. © 2018, Agricultural Research Communication Centre. All rights reserved.
Laboratory of Clinic, Veterinary Medicine Faculty, Syiah Kuala University, Banda Aceh, Indonesia; Laboratory of Reproduction, Veterinary Medicine Faculty, Syiah Kuala University, Banda Aceh, Indonesia; Veterinary Medicine Faculty, Syiah Kuala University, Banda Aceh, Indonesia; Laboratory of Pharmacology, Veterinary Medicine Faculty, Syiah Kuala University, Banda Aceh, Indonesia; Laboratory of Pathology, Veterinary Medicine Faculty, Syiah Kuala University, Banda Aceh, Indonesia
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