Ummu Balqis, Muhammad Hambal, Masda Admi, Safika, Nellita Meutia, Sugito, Dasrul, Mohd. Agus Nashri Abdullah, T. Reza Ferasyi, Triva Murtina Lubis, Mahdi Abrar, Darmawi
Aims: This study aimed to assess the risk of reproductive tract contamination in Aceh cattle by Escherichia fergusonii as revealed by 16S rRNA gene sequencing of preputial swab samples. Methodology and results: Preputial swabs taken from 50 breeding bulls at the Indrapuri Breeding and Forage Center of Aceh Cattle, Banda Aceh, Indonesia, were examined for the presence of bacteria. Samples were streaked on MacConkey agar and incubated under aerobic conditions at 37 °C for 24 h. Smooth, yellow- or rose-colored colonies were selected for their characteristic appearance and subjected to further analysis. Genetic identification was based on 16S rRNA gene sequencing and PCR analysis. We conducted a 16S rRNA sequence similarity search with GenBank using BLAST and constructed neighbour-joining dendrograms using MEGA. From among closely related species of the genus Enterobacteriaceae, we identified the enteric bacterium E. fergusonii as having the highest sequence similarity. Conclusion, significance and impact of study: We concluded that the E. fergusonii bacterium positively presence in preputial swab samples of clinically healthy Aceh cattle population. Accordingly, it is potentially allowing the bacterium to be spread during natural mating or semen collection processing for artificial insemination in cattle breeding farm. © 2018, Universiti Sains Malaysia.
Faculty of Veterinary Medicine, Syiah Kuala University, Jl. Tgk. H. Hasan Krueng Kalee No. 4, Darussalam-Banda Aceh, 23111, Indonesia; Postgraduate Student of Mathematics and Applied Sciences, Syiah Kuala University, Darussalam-Banda Aceh, 23111, Indonesia; Faculty of Veterinary Medicine, Bogor Agricultural University, Jl. Agatis Dramaga-Bogor, 16680, Indonesia; Indrapuri Breeding and Forage Center of Aceh Cattle, Indrapuri District, Banda Aceh, Indonesia; Faculty of Agriculture, Syiah Kuala University, Banda Aceh, Indonesia
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