Isolation and identification of a cellulolytic bacillus from Rumen of Aceh's cattle

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Safika, Wenny Novita Sari, Darmawi, Yudha Fahrimal, Sukmawan Fajar Santosa

2018 Asian Journal of Microbiology, Biotechnology and Environmental Sciences Vol. 20 Issue 3 Article Cited by 4 SDG 2SDG 17 Quartile

Abstract

The purpose of this research was to isolate and identify bacteria that produce cellulase enzyme from rumen fluid of aceh cattle. Biodegradation by cellulase producing bacteria found in rumen can be used as source of cellulolytic bacteria which function to degrade feed material with high coarse fiber so as to improve the quality of nutrition and obtain easily digested feeding materials at a cheaper price than the use of commercial cellulase enzymes. Rumen fluid of aceh's cattle was taken for isolation and screening of cellulolytic bacteria. The FKH-7 isolate showed ± 2.0 cm of clearance zone diameter. Microscopically, this strain was found to be a Gram-positive, Bacillus. Analysis of 16S rRNA and phylogenetic analyses. Homology analysis showed that FKH-7 isolate showed 87% degree of sequence similarity withthat Lysinibacillus fusiformis. This study concluded that some potent cellulose degrading bacteria have been identified in rumen of aceh cattle and that has group Lysinibacillus. © Global Science Publications.

Affiliations

Departement of Veterinary Infection Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Jalan Agatis IPB Dermaga Bogor, Indonesia; Mathematics and Applied Sciences, Syiah Kuala University, Darussalam, Banda Aceh, 23111, Indonesia; Microbiology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Darussalam, Banda Aceh, 23111, Indonesia; Parasitology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Darussalam, Banda Aceh, 23111, Indonesia; Integrated Research Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Darussalam, Banda Aceh, 23111, Indonesia

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