Siska Mellisa, Lionel Dabbadie
The objective of the present study was to evaluate the possibility of using pond water as an alternative media for culturing Nostoc paludosum. Lipid content of N. paludosum using BG II media was investigated. Lipid content was measured by using soxhlet method with petroleum ether as solvent. The highest lipid content (7% of dry weight) was obtained after 11 days, when nitrate concentration is low (451 ppm). Biomass was measured by gravimetric method using Whatman filter paper No. 42. Dry weight of algae was measured after drying in oven at 60°C for 24 hours. Chlorophyll-a was analyzed by using spectrophotometric method. The limiting nutrients of N. paludosum were investigated by using the bioassay method and urea as nitrogen source, K2HPO4 as phosphorus source and CaCO3 as carbon source. Nitrogen is the limiting nutrient. Pond water can be alternative media for culturing N. paludosum although it can produce contamination. © 2015, BIOFLUX SRL. All rights reserved.
Department of Aquaculture, Syiah Kuala University, Banda Aceh, 23111, Indonesia; Aquaculture and Aquatic Resources Management (AARM), Food, Agriculture and Biosystem Cluster (FABS), School of Environment, Resources and Development (SERD), Asian Institute of Technology, P.O. Box 4, Khlong Luang, 12120, Pathumthani, Thailand; CIRAD, Avenue Agropolis, Montpellier Cedex 5, 34398, France
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