Dilla Amalia Bilqis Ikhsan, Natalie Lovena Pramono Putri, Mufasirin Mufasirin, Tita Damayanti Lestari, Ginta Riady, Aswin Rafif Khairullah, Siti Darodjah Rasad, Tjuk Imam Restiadi, Rimayanti Rimayanti, Imam Mustofa, Widjiati Widjiati, Supriyadi Supriyadi, Muhammad Fajar Amrullah, Gabriel Sampe Pasang, Laily 'Ulya Nurul Ilmi, Ahmed Qasim Dawood
High-quality oocyte basis materials are essential for in-vitro embryo formation in animal reproductive biotechnology. Researchers revealed that cow oocytes produced in-vitro can grow and develop. Selection of oocytes is crucial to in-vitro fertilization success and the production of excellent embryos. The aim of study was about the comparison of the number and morphology of oocytes in bovine ovaries obtained through aspiration, slicing and flushing medium techniques. The study used 300 ovaries derived from the remaining slaughtered female cattle in the Surya Slaughterhouse in Pegirian Surabaya. Data obtained included ovarian weight, number of follicles, number and morphology of oocytes, and were then analysed with the SPSS Mann-Whitney test. The results obtained from the study showed that there were differences in the number and morphology of oocytes between aspiration, slicing and flushing medium techniques with a p-value (0.000) < α (0.05). Therefore, H0 was rejected, leading to the conclusion that there were significant differences in the number and morphology of oocytes extracted using between these techniques. The number of oocytes was found to be higher in the slicing method. Morphology of grade A and B oocytes was found to be more prevalent in the aspiration method. © 2011-2025 Journal of Advanced Veterinary Research. All rights reserved.
Profession Program of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, East Java, Surabaya, 60115, Indonesia; Division of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, East Java, Surabaya, 60115, Indonesia; Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, East Java, Surabaya, 60115, Indonesia; Reproduction Laboratory, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Jl. Teuku Nyak Arief, Kopelma Darussalam, Aceh, Banda Aceh, 23111, Indonesia; Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Jl. Raya Bogor, Km. 46 Cibinong, West Java, Bogor, Indonesia; Animal Husbandry Faculty, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM.21, Hegarmanah, Jatinangor, West Java, Sumedang, 45363, Indonesia; Division of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, East Java, Surabaya, 60115, Indonesia; Master Program of Reproductive Biology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, East Java, Surabaya, 60115, Indonesia; Faculty of Animal Science, Universitas Hasanuddin, Jl. Perintis Kemerdekaan No. KM. 10, Tamalanrea Indah, Tamalanrea, South Sulawesi, Makassar, 90245, Indonesia; Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Pendidikan Mandalika, Jl. Pemuda No. 59A, Dasan Agung Baru, West Nusa Tenggara, Mataram, 83125, Indonesia; Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Serdang, 43400 UPM, Malaysia; Department of Physiology, Pharmacology, and Chemistry, Faculty of Veterinary Medicine, Al Shatra University, Al Shatra, Thi Qar, 64007, Iraq
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