Mohd Andalas, Mohammad Hakimi, Detty Siti Nurdiati, Indwiani Astuti, Imran Imran, Harapan Harapan
Background: Single nucleotide polymorphism (SNP) within tumor necrosis factor alpha (TNF-α) gene promoter (-308G/A TNFA) is associated with higher gene expression. The role of this SNP as a risk factor for spontaneous preterm birth has been assessed in some regions and the findings were significantly different between race and ethnic groups. Aim: To provide the scientific evidence whether allele A within SNP -308G/A TNFA promoter is a risk factor for spontaneous preterm birth among Acehnese ethnic or not. Subjects and methods: In this case-control study, the genotypes of SNP -308G/A TNFA among 40 patients with spontaneous preterm birth and 40 patients with term birth were determined by real-time polymerase chain reaction (RT-PCR). The concentrations of TNF-α from blood were measured by enzyme-linked immunosorbent assay (ELISA). The differences in genotype distributions, dominant and recessive models, and allele frequencies between case and control groups were analyzed with Chi-squared test. Deviation of genotype frequencies from the Hardy-Weinberg equilibrium (HWE) was assessed by Fisher's exact test. Results: This study found that the concentration of TNF-α between preterm and control groups was not statistically different, 5.5 ± 2.9 mg/dL vs. 10.1 ± 17.9 mg/dL, p = 0.112. The level of TNF-α had no strong association with either genotype distribution or allele frequency of SNP -308G/A TNFA. Furthermore, there was no association between mutant genotypes and spontaneous preterm birth (OR: 0.32; 95%CI: 0.08-1.33, p = 0.096) and between mutant allele and spontaneous preterm birth (OR: 0.35; 95%CI: 0.09-1.37, p = 0.105). Conclusion: SNP -308G/A TNFA is not associated with spontaneous preterm birth in Acehnese ethnic group. © 2015 The Authors.
Department of Obstetrics and Gynecology, School of Medicine, Syiah Kuala University, Banda Aceh, 23111, Indonesia; Department of Obstetrics and Gynaecology, Faculty of Medicine, Gadjah Mada University, Yogyakarta, 55281, Indonesia; Department of Pharmacology, Faculty of Medicine, Gadjah Mada University, Yogyakarta, 55281, Indonesia; Medical Research Unit, School of Medicine, Syiah Kuala University, Banda Aceh, 23111, Indonesia
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